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Fig. 2 | BMC Cancer

Fig. 2

From: An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1

Fig. 2

DBID screening of a small molecule chemical library (1685 compounds) identifies inhibitors of CGGBP1-DNA interaction. a: The primary DBID assay was performed in multiple 96-well plates. The lysate was individually pre-incubated with the compounds (one compound per well). After transferring the lysate-compound complexes (as shown in Fig. 1c) to dot blots (as shown in Fig. 1a), immunochemical detection was performed using a cocktail of rabbit anti-CGGBP1 primary antibody. The schematic represents the signals obtained for a 96-well plate. b: The dot blots of the entire library screen for CGGBP1 were manually categorized into 11 strong inhibitors and 20 moderate inhibitors. The actual images of these two groups of dot blots are shown here along with the positive and negative controls as indicated. The identities of the inhibitors are as follows: Strong inhibitors [A1-Givinostat (ITF2357), A2-LRRK2-IN-1, A3-Peficitinib (ASP015K, JNJ-54781532), B1-Ispinesib (SB-715992), B2-TWS119, B3-Domperidone, C1-Gallamine Triethiodide, C2-Moxifloxacin HCl, C3-Sirtinol D1-NAD+, D2-Palbociclib (PD-0332991) HCl], Moderate inhibitors [A5-VX-661, A6-BRD73954, A7-NCT-501, A8-Tenovin-1, A9-Prasugrel, B5-U0126-EtOH, B6-Foretinib (GSK1363089), B7-JNJ-7706621, B8-CHIR-99021 (CT99021), B9-Asenapine maleate, C5-Ethylparaben, C6-LY2874455, C7-Golgicide A, C8-PD173955, C9-Mirin, D5-Ramelteon, D6-Cilnidipine, D7-Dopamine HCl, D8-VR23, D9-AZD3759]. Majority of the compounds in the library did not show any inhibition of CGGBP1-DNA interaction. Thirty representative dot blots of the non-inhibitors are shown in the well numbers F1-F10, G1-G10, H1-H10. c: The signals for the dot blots shown in B are quantified by densitometry. The graph shows the signals of the inverted images for each dot blot

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