Fig. 5

Induction of DNA damage in non-cancerous ovarian and fallopian epithelium upon estrogen exposure. a-b, Representative images for 53BP1 and γH2AX foci (a) and quantifications for 53BP1 positivity (b) in OCT-embedded ovary and liver cryosections. Mice were pre-treated with intraperitoneal injection of estrogen (1 mg/Kg) and sacrificed after 6 h. Dramatic induction of 53BP1/γH2AX foci was observed in epithelial ovary instead of hepatocytes. Pairs of samples from 4 mice were analyzed. c, Images (left) and quantification for 53BP1-positive cells (right) for immunostainings for 53BP1 and γH2AX foci in fallopian tube from same mice of (a-b). Enlarged areas showing differential foci morphology in epithelial layer (Epi) and smooth muscle (SM). d, Comparison for 53BP1 foci number per cell in Epi versus SM compartments. p values: unpaired t-test between SM and Epi. n = 4