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Fig. 1 | BMC Cancer

Fig. 1

From: Cell type-specific genotoxicity in estrogen-exposed ovarian and fallopian epithelium

Fig. 1

Uncoupled elevation of DNA damage and genomic replication in ovarian cancer cells. a, Indirect immunofluorescent (IF) staining of endogenous foci formation of indicated repair factors in OVCAR-8TR and RPE1 cells (upper panel) and quantifications for positive cells (lower panel). 53BP1, RPA32-pSerine 33 and BRCA1-pSerine1524 were monitored by specific antibodies. ***: p < 0.001, two-tailed unpaired t-test. Three biological replicates were analyzed. b, Representative images (upper) and quantification (lower) for 53BP1-positive cells (> 3 per cell) in primary cells isolated from three independent OvCa biopsies after culturing in RPMI1640/Matrigel for 48 h. BrdU-positive cells were quantified in parallel. Data showed as means ± SEM of 3 independent assays

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