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Table 1 Primer sequences used in RT-qPCR

From: microRNA-451a promoter methylation regulated by DNMT3B expedites bladder cancer development via the EPHA2/PI3K/AKT axis

Targets

Forward (5′-3′)

Reverse (5′-3′)

miR-451a

ACACTCCAGCTGGGAAACCGTTACCATTAC

CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTTACAG

DNMT3B

TAACAACGGCAAAGACCGAGGG

TCCTGCCACAAGACAAACAGCC

EPHA2

ACTGCCAGTGTCAGCATCAACC

GTGACCTCGTACTTCCACACTC

U6

AAAGCAAATCATCGGACGACC

GTACAACACATTGTTTCCTC

GAPDH

CTGGGCTACACTGAGCACC

AAGTGGTCGTTGAGGGCAATG

  1. Note: RT-qPCR quantitative reverse transcriptase-PCR, miR microRNA, DNMT3B DNA methyltransferase 3B, EPHA2 erythropoietin-producing hepatocellular receptor tyrosine kinase class A2, GAPDH glyceraldehyde-3-phosphate dehydrogenase
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BMC Cancer

ISSN: 1471-2407

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