Fig. 6

miR-451a-mediated EPHA2 silencing inhibits malignant biological behavior of BCa cells. T24 and 5637 cells were transfected with miR-451a mimic alone or in the presence of oe-EPHA2. a, miR-451a expression and the mRNA expression of EPHA2 in BCa cells after transfection assessed by RT-qPCR; b, the number of BCa cells in the S phase determined by EdU staining where red represents the replicating DNA stained by EdU and blue represents the nucleus stained by DAPI; c, apoptosis in T24 and 5637 cells detected by flow cytometry; d, number of migratory BCa cells assessed by Transwell assays; e, number of invasive T24 and 5637 cells was detected by Transwell assays; f, the extents of PI3K and AKT phosphorylation in T24 cells (full-length blots/gels are presented in Supplementary Figure 6A-C) and 5637 cells (full-length blots/gels are presented in Supplementary Figure 7A-C) determined by western blot. Data are represented as the means ± SD from three independent assays. Statistically significant differences were calculated using one-way or two-way ANOVA, followed by Tukey’s multiple comparison test. **p < 0.01 vs Mock or miR-451a + oe-NC treatment