Fig. 1

Co-culture with HGSOC cell lines promoted the differentiation and polarization of primary human macrophages towards the alternatively activated macrophage (AAM) phenotype. a Immunofluorescent detection of CD68 (a macrophage marker, green) and CD163 (an AAM marker, green) in monocytes that have been co-cultured with HGSOC cell lines for 7 days. Cells were counterstained with DAPI (blue). Scale bar = 250 μm. b-e Percentage of cells that were CD68+ (b), number of CD68+ cells per field of view (C), percentage of cells that were CD163+ (d), and number of CD163+ cells per field of view (e) after monocytes have been co-cultured with HGSOC cell lines for 7 days. Data are expressed as average ± SD, n = 3 monocyte donors. Groups with no statistically significant differences are linked by the same letters, while groups that are statistically different from each other have different letters, p < 0.05 by Tukey-HSD