Cytokeratin-positive cells in the bone marrow from patients with pancreatic, periampullary malignancy and benign pancreatic disease show no prognostic information

Table 3 Published studies on DTC status in the bone marrow for peri-ampullary adenocarcinomas, grouped by detection method

Authors	Method/ Markers	Detection limit	DTC def.	Pat. number	Loc. / Adv.	TC-rate	false-pos. Rate	Prog.	Comments
Immunocytochemistry
Effenberger 2012 [13]	CK A45-B/B3	≥1 / 2 × 10⁶ MNC	TC	n = 175	n = 71 / n = 104	13.7% (24/175)	–	Pos.	29 pat. Resected despite advanced cancer stage
Rehders 2012 [25]	CK A45-B/B3	≥1 / 1 × 10⁶ MNC	TC	n = 49	n = 49 /-	27% (12/49)	–	Neg.	No details on survival analysis disclosed
Roder 1999 [17]	CK 2; KL1; A45-B7B3	≥1 / 5 × 10⁵ MNC	TC	n = 48	n = 8 / n = 40	52.1% (25/48)	–	Pos.	Association with OS for resected patients
van Heek 2001 [16]	CK (8, 18)	≥1 / 1 × 10⁷ MNC	TC	n = 35	n = 14 / n = 13	34.2% (12/35)	50%(2/4)	Pos.	2/4 benign pat. False positive
Vogel 1999 [14]	panCK, mucin, CEA, Ca-19–9	≥1 / 1.25 × 10⁶	TC	n = 80	n = 11 / n = 60	38%(27/71)	6.6%(3/45)	Pos.
Thorban 1996 [15]	CK A45-B/B3	≥1 / 5 × 10⁵ MNC	TC	n = 42	n = 24 / n = 18	57.1% (24/42)	–	Pos.	Association with metastatic and local relapse
Z’Graggen 2001 [26]	CK AE3/AE1	≥1 / 5 × 10⁶ MNC	TC	n = 54	n = 3 / n = 51	24% (13/54)	4.1%(1/24)	Neg.
Nucleotide based detection
Hoffmann 2007 [24]	rt-PCR / CK-19	–		n = 37	n = 7 / n = 30	67%(25/37)	–	Neg.
Soeth 2005 [23]	rt-PCR / CK-20	–		n = 117	n = 172	33.3%(45/135)	11%	Neg.	Survival data only for blood/BM combined. False positive rate 11%

Prog Prognostic impact of DTC-status on survival Loc Localised cancers; Adv Advanced cancers; MNC Mononuclear cells

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