Gene | Sequence |
---|---|
CHN1 3’UTR | Forward: 5′-(C)TTGAGGGGAAAAGAAATG-3’ |
Reverse: 5′-ATGTAACAGCCAGAGGTGC-3’ | |
CHN1-mut | Forward: 5′-AGAAATGTTTTACAGGCTGGCCGATGTTTTATAG-3’ |
Reverse: 5′-CGGCCAGCCTGTAAAACATTTCTTTTCCCCTCA-3’ | |
Has-miR-205 | RT-205: 5′-GTCGT ATCCA GTGCA GGGTC CGAGG TATTC GCACT GGATA CGACC AGACT-3’ |
Forward: 5′-AATTGTCCTTCATTCCACCGG-3’ | |
Reverse: 5′-GTGCAGGGTCCGAGGT-3’ | |
Human U6 | Forward: 5′-CGCTTCGGCAGCACATATAC-3’ |
Reverse: 5′-TTCACGAATTTGCGTGTCAT-3’ | |
CHN1 | Forward: 5′-GGAGCTACCTCATCCGGGAG-3’ |
Reverse: 5′-TGTGTCTCTTTCAGGACTGGCA-3’ | |
GAPDH | Forward: 5′-GGTCTTACTCCTTGG AGGCCATGTG-3’ |
Reverse: 5′-ACCTAACTACATCGTTTACATGTT-3’ | |
LNA-miR-205 probe | 5′-CAG(+A)C(+T)CCGG(+T)GGAA(+T)GA(+A)GGA-Dig-3’ |
Hsa-miR-205 | 5′-UCCUUCAUUCCACCGGAGUCUG-3’ |
Hsa-miR-205 mimic | 5′-GACUCCGGUGGAAUGAAGGAUU-3’ |
MircoRNA inhibitor NC | 5′-CAGUACUUUUGUGUAGUACAA-3’ |
Hsa-miR-205 inhibitor | 5′-CAGACUCCGGUGGAAUGAAGGA-3’ |
Negative control | 5′-UUCUCCGAACGUGUCACGUTT-3’ |
si-CHN1 | 5′-GGCUUGAUUACUCUCUAUATT-3’ |