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Table 2 The sequences of siRNA, miRNA and primer used in this study

From: Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis

Gene Sequence
CHN1 3’UTR Forward: 5′-(C)TTGAGGGGAAAAGAAATG-3’
Reverse: 5′-ATGTAACAGCCAGAGGTGC-3’
CHN1-mut Forward: 5′-AGAAATGTTTTACAGGCTGGCCGATGTTTTATAG-3’
Reverse: 5′-CGGCCAGCCTGTAAAACATTTCTTTTCCCCTCA-3’
Has-miR-205 RT-205: 5′-GTCGT ATCCA GTGCA GGGTC CGAGG TATTC GCACT GGATA CGACC AGACT-3’
Forward: 5′-AATTGTCCTTCATTCCACCGG-3’
Reverse: 5′-GTGCAGGGTCCGAGGT-3’
Human U6 Forward: 5′-CGCTTCGGCAGCACATATAC-3’
Reverse: 5′-TTCACGAATTTGCGTGTCAT-3’
CHN1 Forward: 5′-GGAGCTACCTCATCCGGGAG-3’
Reverse: 5′-TGTGTCTCTTTCAGGACTGGCA-3’
GAPDH Forward: 5′-GGTCTTACTCCTTGG AGGCCATGTG-3’
Reverse: 5′-ACCTAACTACATCGTTTACATGTT-3’
LNA-miR-205 probe 5′-CAG(+A)C(+T)CCGG(+T)GGAA(+T)GA(+A)GGA-Dig-3’
Hsa-miR-205 5′-UCCUUCAUUCCACCGGAGUCUG-3’
Hsa-miR-205 mimic 5′-GACUCCGGUGGAAUGAAGGAUU-3’
MircoRNA inhibitor NC 5′-CAGUACUUUUGUGUAGUACAA-3’
Hsa-miR-205 inhibitor 5′-CAGACUCCGGUGGAAUGAAGGA-3’
Negative control 5′-UUCUCCGAACGUGUCACGUTT-3’
si-CHN1 5′-GGCUUGAUUACUCUCUAUATT-3’