Skip to main content

Table 2 The sequences of siRNA, miRNA and primer used in this study

From: Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis

Gene

Sequence

CHN1 3’UTR

Forward: 5′-(C)TTGAGGGGAAAAGAAATG-3’

Reverse: 5′-ATGTAACAGCCAGAGGTGC-3’

CHN1-mut

Forward: 5′-AGAAATGTTTTACAGGCTGGCCGATGTTTTATAG-3’

Reverse: 5′-CGGCCAGCCTGTAAAACATTTCTTTTCCCCTCA-3’

Has-miR-205

RT-205: 5′-GTCGT ATCCA GTGCA GGGTC CGAGG TATTC GCACT GGATA CGACC AGACT-3’

Forward: 5′-AATTGTCCTTCATTCCACCGG-3’

Reverse: 5′-GTGCAGGGTCCGAGGT-3’

Human U6

Forward: 5′-CGCTTCGGCAGCACATATAC-3’

Reverse: 5′-TTCACGAATTTGCGTGTCAT-3’

CHN1

Forward: 5′-GGAGCTACCTCATCCGGGAG-3’

Reverse: 5′-TGTGTCTCTTTCAGGACTGGCA-3’

GAPDH

Forward: 5′-GGTCTTACTCCTTGG AGGCCATGTG-3’

Reverse: 5′-ACCTAACTACATCGTTTACATGTT-3’

LNA-miR-205 probe

5′-CAG(+A)C(+T)CCGG(+T)GGAA(+T)GA(+A)GGA-Dig-3’

Hsa-miR-205

5′-UCCUUCAUUCCACCGGAGUCUG-3’

Hsa-miR-205 mimic

5′-GACUCCGGUGGAAUGAAGGAUU-3’

MircoRNA inhibitor NC

5′-CAGUACUUUUGUGUAGUACAA-3’

Hsa-miR-205 inhibitor

5′-CAGACUCCGGUGGAAUGAAGGA-3’

Negative control

5′-UUCUCCGAACGUGUCACGUTT-3’

si-CHN1

5′-GGCUUGAUUACUCUCUAUATT-3’