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Fig. 7 | BMC Cancer

Fig. 7

From: Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis

Fig. 7

Knockdown of CHN1 attenuated the miR-205-mediated enhancement of cell growth and metastasis. a SiHa cells were transfected with NC or si-CHN1. At 48 h after transfection, CHN1 mRNA expression was determined by qRT-PCR. GAPDH served as an internal reference gene. b CHN1 protein levels were determined by western blotting at 48 h after transfection. β-Actin was used as a loading control. The cropping of the blot was done. Full-length uncropped blots are presented in Supplementary Figure 2, which all the samples derived from the same experiment and blots were processed in parallel. c Cell proliferation was determined by CCK-8 assay at 48 h after transfection. SiHa cells were transfected with NC, si-CHN1, or miR-205 mimic or cotransfected with miR-205 mimic and si-CHN1. d Apoptosis was detected by flow cytometry. The percentages of early and late apoptotic cells (representative of three separate experiments) are shown in the lower right and upper right panels, respectively. e Cells (0.5 × 106 cells/mL) were subjected to migration or invasion assays as described in the methods. Magnification, 100×; scale bar =200 μm. *P < 0.05

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