Fig. 1

NFAT2 overexpression suppressed cell viability and induced cell apoptosis in HepG2 cells. a Western blot and qRT-PCR analysis of protein and gene expression of NFAT2 in HepG2 cells transfected with pcDNA3.1-NFAT2 and pcDNA3.1 plasmids (n = 3). b The cell viability of NC and HepG2/NFAT2 cells was represented by absorbance values (λ = 450 nm), detected by CCK-8 kit (n = 6). c The cell apoptosis assay of NC and HepG2/NFAT2 cells by Annexin V-FITC and PI staining (n = 6). d The cell proliferation assay of NC and HepG2/NFAT2 cells by EdU labeling (n = 6). Each bar represents the mean ± S.D.; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared to the NC samples. Full-length blots are presented in Supplementary Figures