Table 2 Sequences of luciferase reporter plasmid construct
From: miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1
Primer | Sequence |
|---|---|
IRS-1 site A F | 5′-TCTAGAGACCTCAGCAAATCCTCTTCTA-3’ |
IRS-1 site A R | 5′-TCTAGAAAGGTTGAAGATGAAGTTTATGC-3’ |
IRS-1 site B F | 5′-TCTAGAGCTGGTTTTGATGGTGGCA-3’ |
IRS-1 site B R | 5′-TCTAGAAACGCTGTGAGAGGTTGGTG-3’ |
IRS-1-site A-Mut1 | 5′- CGATGCATCAGATCTCGTTTGT − 3’ |
IRS1-site A-Mut2 | 5′- ACAAACGAGATCTGATGCATCG − 3’ |
IRS1-site A-Del1 | 5′-GTACGATGCATCGTTTGTTTAC-3’ |
IRS1-site A-Del2 | 5′-GTAAACAAACGATGCATCGTAC-3’ |
IRS1-site B-Mut1 | 5′-GGCTTTTATCAGATCTCAAGCA-3’ |
IRS1-site B-Mut2 | 5′- TGCTTGAGATCTGATAAAAGCC −3’ |
IRS1-site B-Del1 | 5′-CTTTTATCAAGCATTTGTAGGCCA-3’ |
IRS1-site B-Del2 | 5′-TGGCCTACAAATGCTTGATAAAAG-3’ |