Fig. 7

miR-203 inhibits proliferation and migration by targeting IRS-1. a-b Cell proliferation were detected with CCK8 assay in PC-3/DU145-mir-203 (+pEGFP/+IRS-1) cells cultured for 72 h. pCDH/pEGFP was used as an empty control. Data represent mean ± SD with three replicates, *P < 0.05, ***P < 0.001, **** P < 0.0001. c Cell proliferation were detected by colony formation assay after restoration of IRS-1 expression in DU145-miR-203 and PC-3-miR-203 cell cultured for 12–14 days. d-g Migration of PCa-miR-203 cells overexpressing IRS-1 or a vector control was analyzed through wound-healing assay. Cells were examined by light microscopy at indicated time points. Scale bar = 200 μm. h The expression of E-cadherin, Vimentin and Slug was detected after restoration of IRS-1 expression in DU145-miR-203 and PC-3-miR-203 cells. pCDH/pEGFP was used as an empty control. β-Tubulin serves as internal control. The level of IRS-1 and other proteins indicated in figure were quantified with Image J software and normalized to the internal control. The full-length blots of panel 7 h were presented in Supplementary Figure 4