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Fig. 6 | BMC Cancer

Fig. 6

From: miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1

Fig. 6

Both miR-203 overexpression and IRS-1 knockdown inhibit PCa cell migration. a Analysis of the GDS4114 from the GEO database microarray (containing 12 samples, 6 normal prostate matrix samples and 6 invasive prostate cancer matrix samples), * P < 0.05. b-e PCa cells knocking down IRS-1 or a vector control were analyzed by wound-healing assay. Cells were examined by light microscopy at indicated time points. Scale bar = 100 μm. * P < 0.05, ** P < 0.01. f-i PCa cells overexpressing miR-203 or a vector control were analyzed by wound-healing assay. Cells were examined by light microscopy at indicated time points. Scale bar = 100 μm. * P < 0.05. j Trans-well migration assay analysed the ability of migration in PCa cells with miR-203 overexpression. Cells were examined by light microscopy at indicated time points. Scale bar = 200 μm. k Trans-well migration assay analysed the ability of migration in PCa cells with IRS1 knockdown. Cells were examined by light microscopy at indicated time points. Scale bar = 200 μm. l The expression of E-cadherin, Vimentin and Slug was detected after knocking down IRS-1. β-Tubulin serves as internal control. The level of IRS-1 and other proteins indicated in figure were quantified with Image J software and normalized to the internal control. The full-length blots of panel 6 l were presented in Supplementary Figure 3

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