Fig. 5

miR-203 inhibits ERK signalling pathway by targeting IRS-1. a, b Protein expression levels was detected by Western Blot after knocking down IRS-1 in DU145 and PC-3. c, d Protein expression levels was detected by Western Blot after overexpressing miR-203 in DU145 and PC-3. e, f IRS-1 was over-expressed in DU145-miR-203 and PC-3-miR-203 cell lines stably expressing miR-203, pEGFP was used as an empty control. Changes in protein expression levels were analyzed by Western Blot. β-tubulin serves as internal control. The level of IRS-1 and other proteins indicated in figure were quantified with Image J software and normalized to the internal control. The full-length blots images of figure (5a-5f) were shown in Supplementary Figure 2