Fig. 4
From: miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1
miR-203 induces cell cycle arrest at G0/G1 phase by down-regulating IRS-1. a Changes in cell cycle distribution were analyzed by flow cytometry after knocking down IRS-1 in DU145 and PC-3. Data represent mean ± SD with three replicates, ns: not significant, * P < 0.05, ** P < 0.005, *** P < 0.005. b Changes in cell cycle distribution were analyzed by flow cytometry after overexpression of miR-203 in DU145 and PC-3. Data represent mean ± SD with three replicates, ns: not significant, * P < 0.05, ** P < 0.005. c IRS-1 was over-expressed in DU145-miR-203 and PC-3-miR-203 cell lines stably expressing miR-203, pEGFP was used as an empty control. Changes in cell cycle distribution were analyzed by flow cytometry. Data represent mean ± SD with three replicates, ns: not significant, * P < 0.05, ** P < 0.005