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Fig. 5 | BMC Cancer

Fig. 5

From: KIAA0101 and UbcH10 interact to regulate non-small cell lung cancer cell proliferation by disrupting the function of the spindle assembly checkpoint

Fig. 5

KIAA0101 inhibits the binding of UbcH10 and APC/ C by KEN-box. a Construction of the KIAA0101 vector with wild type and mutant KEN-box sites. Left, KEN-box locus and mutation information; right, KEN-box locus sequence analysis; b binding of UbcH10 and APC/C was detected by immunoprecipitation. Left, the cells were transfected with the KIAA0101 expression vector containing the wild type KEN-box site, pcDH-KIAA0101 (KEN-wt); right, the cells were transfected with the KIAA0101 expression vector containing the mutant KEN-box site, pcDH-KIAA0101 (KEN-mt). The sample was collected 48 h after transfection. The target APC/C band size was 160 kDa

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BMC Cancer

ISSN: 1471-2407

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