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Fig. 2 | BMC Cancer

Fig. 2

From: KIAA0101 and UbcH10 interact to regulate non-small cell lung cancer cell proliferation by disrupting the function of the spindle assembly checkpoint

Fig. 2

Correlation of UbcH10 and KIAA0101 expression in NSCLC cells and tissues. a The temporal correlation between UbcH10 and KIAA0101 in SK-MES-1cells as detected by western blotting. Cells were arrested in the G2 phase with Nocodazole (1 μg/ml) and then harvested at 0, 1, 2, 3, 4 and 5 h after release into the medium. Total proteins were extracted, and UbcH10 and KIAA0101 expression was detected by western blotting with β-actin serving as a reference, the relative expression of the target protein was expressed as the optical density ratio of the target protein to β-actin. Low, scanned target protein bands, top, the protein expression curve over time. b The spatial correlation between UbcH10 and KIAA0101 in SK-MES-1 cells as detected by immunofluorescence is shown as follows: cell nuclei are stained blue by Hoechst, and green and red labels show UbcH10 and KIAA0101 proteins, respectively. The magnification was 200×. c The spatial correlation between UbcH10 and KIAA0101 in NSCLC tissues as detected by immunohistochemistry is shown. Five fields were randomly selected for comparison

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