Fig. 4

Decreased tumor burden and M2 macrophages following NF-κB activation in macrophages during tumor implantation. FVB IKFM and control mice were treated with 1 g/L dox from 3 days before TBR5 cell injection until 7 days post-injection (in red). Mice were sacrificed 14 days after being taken off dox. a Schematic of experimental design. b Harvested omental tumor weight and c ascites volume at sacrifice. Values are mean + SEM of n = 10 per group. d Representative low power 4x magnification H&E images of control and IKFM tumors. Semi-automated counts of e percent of F4/80 positive cells per high-powered field (HPF), f percent of Arg-1 positive cells per HPF, and g the ratio of Arg-1⁺ to F4/80⁺ cells. Counts were quantified from 5 high power fields from each representative sample and represent percentage of cell subtype relative to total cellularity in each field. h Representative images of tumors stained for F4/80 (macrophages; green), arginase-1 (M2 macrophage marker; Arg-1; red), and DAPI (nuclei; blue). Values are mean + SEM of n = 3 for each group; P-values are from the Mann-Whitney test. Figure 4a was generated with Microsoft PowerPoint 2016 (Version 16.16.26); Fig. 4b, c, and e-g were generated with GraphPad Prism (Version 8: La Jolla, California, USA); and Fig. 4d and h were generated with Adobe Photoshop CC 2018 (Version 19.0.1)