Fig. 3

Shift from M2 towards M1 phenotypes in ascites cells from IKFM mice. FVB IKFM and control mice injected with TBR5 cells were treated with 1 g/L Dox from 7 to 21 days post-tumor cell injection, with a two-day break from days 12–14. In harvested ascites cells, mRNA levels of the following were measured by qRT-PCR analysis: a epithelial tumor marker CK18, b pan macrophage marker F4/80, c-f M1 macrophage markers (CD38, CCL3, iNOS, and TNFα), g-i M2 macrophage markers (mannose-receptor, Egr2 and IL-10). Values were calculated using the 2^-ΔΔCt method relative to corresponding levels of the B2M a-c, g-i or GAPDH d-f internal control. Values are shown in log2 scale and are mean + SEM (*p < 0.05, **p < 0.005, ***p < 0.001 relative to control, Mann-Whitney test). j VEGF levels in the soluble fraction of ascites from FVB IKFM mice were measured by ELISA. Values were expressed relative to corresponding protein content and represent mean + SEM for each group measured in duplicate (n = 3 control, n = 4 IKFM). Figure 3a-j were generated with GraphPad Prism (Version 8: La Jolla, California, USA)