Fig. 4

Effects of acteoside and sorafenib on angiogenesis in vitro. a and c, Matrigel tube-formation assay of HUVECs. Cells were treated with acteoside (A01; 100 or 500 μM) and sorafenib (sora; 10 μM) alone or in combination. Tube formation was then evaluated at 3 h and 8 h post-treatment. b and d, Wound healing assay in HUVECs. The cell layer was scratched with a pipette tip. Cells were then treated with acteoside (A01; 100 or 500 μM) and sorafenib (sora; 10 μM) alone or in combination. Cell migration was visualized at the indicated time point (0 and 10 h) under an inverted light microscope at a magnification of × 100. Scale bar, 500 μm. Data are expressed as mean ± SD from three independent experiments. **,p < 0.01 vs the control group or otherwise as specified