Fig. 2

Effects of acteoside and sorafenib on colony formation of HCC cells. Cells were treated with acteoside (100 μM), sorafenib (2 μM) or their combination after seeded in 24-well plates (200 cells/well) overnight. Culture medium was replaced every 3 days. Colonies counting was performed after 12 days of culturing under a light microscope. Data are expressed as mean ± SD from three independent experiments. *,p < 0.05, **,p < 0.01