Targeted next-generation sequencing assays using triplet samples of normal breast tissue, primary breast cancer, and recurrent/metastatic lesions

Table 1 Clinicopathological factors of the breast cancer patients with recurrent/metastatic lesions

Case ID	Primary or relapse	Age	Primary systemic therapy	Adjuvant therapy	Histology	pT	pN	Grade	Ly	V	ER (%)	PgR (%)	HER2	Ki67 (%)	Follow-up (months)	Outcome
K18	breast primary	41	CPA, EPI, DTX, 5’DFUR	X	ICNOS	1a	0	2	0	1	0	0	0	76.0	23	DOD
K18	local recurrence	42									< 1	0	1+	70.5
K20	breast primary	37	CPA, EPI	PTX, TRA, TAM	ICNOS	2	2a	3	3	1	60	0	3+	57.1	60	DOD
K20	pleural effusion	42									0	0	2 + (FISH +)	45.0
K21	breast primary	66	CPA, EPI, EXE	X, EXE	ICNOS	4b	1a	2	1	2	90	10	1+	39.5	63	DOD
	local recurrence	68									100	70	1+	22.3
	local recurrence	69									100	90	1+	47.6
K22	breast primary	63	no	TAM	ILC	1b	0	1	0	0	30	0	0	9.4	25	DOD
K22	pleural effusion	65									0	< 1	0	24.8
K23	breast primary	65	no	no	ICNOS	1a	0	1	0	0	95	80	1+	11.8	92	LND
K23	local recurrence	68									95	90	1+	5.8
K25	breast primary	35	CPA, EPI, DTX, 5’DFUR	GEM	ICNOS	2	1a	3	1	1	0	0	0	55.4	21	DOD
K25	local recurrence	36									0	0	1+	83.0
K27	breast primary	55	CPA, EPI	X	ICNOS	3	0	3	3	0	5	2	1+	68.2	7	DOD
K27	local recurrence	55									0	0	0	87.0
K28	breast primary	50	CPA, EPI, DTX, 5’DFUR	CPA, EPI, X	ICNOS	3	1a	3	3	2	0	0	1+	86.8	50	DOD
K28	local recurrence	51									0	0	1+	57.4
K30	breast primary	68	no	5FU, CPA, EPI	ICNOS	1c	3b	3	1	0	0	0	0	80.2	12	DOD
	pleural effusion	68									0	0	0	87.0
	pleural effusion #2	69									0	0	0	84.0
K31	breast primary	47	CPA, EPI	CPA, X	ICNOS	1a	3a	3	1	0	0	0	0	65.4	28	DOD
	pericardial effusion	48									0	0	0	36.6
	pleural effusion	49									0	0	0	58.4
K32	breast primary	38	no	CPA, EPI, TAM	ICNOS	1c	1mi	2	2	1	80	2	1+	37.5	83	DOD
K32	lung metastasis	45									10	0	0	58.4

CPA cyclophosphamide, EPI epirubicin, DT docetaxel, 5’DFUR doxifluridine, 5FU 5 fluorouracil, TAM tamoxifen, X capecitabine, PTX paclitaxel, TRA trastuzumab,
EXE exemestane, GEM gemcitabine, ICNOS invasive carcinoma of no special type, ILC invasive lobular carcinoma, na not available, DOD dead of disease, LND living without disease

ISSN: 1471-2407