Fig. 3

Phosphorylation of PDH was increased in SUM149 INV compared to WCC Expression of pPDH S300, pPDH S232, pPDH S239, PDH, and GAPDH proteins of INV and WCC were examined with western blot analysis and bands were shown in (a), and the graphs of phosphorylated-PDH vs. pan-PDH were shown in (b). Data are presented as mean ± SD of samples (n = 3). *P < 0.05, **P < 0.01 vs. WCC. Uncropped full-length blots images were shown in Supplemental Figure 1. c Glucose uptake into WCC or INV was examined with 2-NBDG, and analyzed with flow cytometer. Live cells were gated as P1 (c), and levels of FITC and mean fluorescent intensity (MFI) was shown in graph (d), and (e), respectively. 2-NBDG represents a fluorescent tracer used for monitoring glucose uptake, and Phlo represents phloretin, inhibitor for the glucose transporter, respectively. Data are presented as mean ± SD of samples (n = 3). **p < 0.01, ***p < 0.001