Fig. 1

SUM149 was selected for collecting invaded cells from transwell inserts. a Invasion assay was performed with using seven human breast cancer cell lines, MCF-7, BT-474, SK-BR-3, MDA-MB-468, MDA-MB-231, HCC1937, and SUM149. Representative images of invaded cells reached underneath of the transwell membrane are shown. Scale bar: 200 μm. b Percent of invaded cells of seven human breast cancer cell lines is shown in graph. Data are presented as mean ± SDs of triplicate samples. c Whole cultured SUM149 cells (WCC) and INV collected from underneath of transwell membranes were used for invasion assay. Number of invaded cells were counted, and ratio of invaded cells of INV group to WCC group was summarized in graph. Data are presented as mean ± SDs of triplicate samples. *p < 0.05, **p < 0.01