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Table 3 Comparison of SEQ and HRM detected in Exon 19 and 21 mutation positive samples

From: Somatic mutation detection efficiency in EGFR: a comparison between high resolution melting analysis and Sanger sequencing

HRM Detected SEQ Detected p value Sensitivity Specificity PPV NPV Accuracy
Positive (%) Negative (%)
Positive (Exon 19) 47 (72.3) 18 (27.7) <‚ÄČ 0.001 100% 74% 72.3% 100% 84.4%
Negative (Exon 19) 0 (0) 51 (100)
Positive (Exon 21) 15 (60) 10 (40) 0.002 100% 90.1% 60% 100% 91.3%
Negative (Exon 21) 0 (0) 91 (100)