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Table 1 Mean Ct obtained using allele-specific probe Real time PCR

From: Somatic mutation detection efficiency in EGFR: a comparison between high resolution melting analysis and Sanger sequencing

MAF (%) MutDNA (FAM)
(mean ± sd)
wtDNA (VIC)
(mean ± sd)
ΔCt (mutDNA-wtDNA)
(mean ± sd)
50 24.35 ± 0.32 19.27 ± 0.39 5.08 ± 0.29
10 24.03 ± 0.56 18.55 ± 0.47 5.47 ± 0.56
5 25.18 ± 0.27 19.01 ± 0.28 6.17 ± 0.36
1 30.83 ± 0.91 20.23 ± 0.28 10.6 ± 1.01
0.1 34.26 ± 0.89 20.37 ± 0.29 13.89 ± 1.15
WT 38.33 ± 2.89 22 ± 0.01 16.33 ± 2.89