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Fig. 5 | BMC Cancer

Fig. 5

From: Somatic mutation detection efficiency in EGFR: a comparison between high resolution melting analysis and Sanger sequencing

Fig. 5

The relationship between the change in Ct value and concentration of DNA per assay. Log Dilution curve for 50% MAF sample showing Ct value difference (y-axis) to negative log of DNA concentration, −ln [DNA/assay], from 0.25 ng/μl to 0.5 pg/μl (x-axis). The trendline in the graph shows the correlation between -ln [DNA/assay] and ΔCt value (ΔCt value = Ct [at given DNA conc.] – Ct [maximum DNA conc.]). The inset of the graph shows the slope of the trendline and correlation (R2 value = 0.99). The relationship between ΔCt value and concentration of DNA can be obtained from the slope of the graph

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