Fig. 6
Extrapolation of the protein-based cell cycle and apoptosis model responses to drug exposure. PANC-1 cell proliferation responses to vehicle (control; P0B0) and different concentrations of BRP/PTX combinations were simulated using the final cell cycle and apoptosis model and compared with experimentally-observed values for cell proliferation over 120 h of exposure (Supplementary Fig. S1). Adjustments to the model parameters, such as increasing Nmax (the capacity of live cells in the Gompertz function) to 30 and decreasing kma0 (the rate of cycling G2/M cells transition to mitotically-arrested cells) to 1.67 × 10− 2 h− 1 were made to account for different culture conditions, as described in the text. a Representative simulations overlaid on observed cell proliferation data. b Simulated (Sim) vs. observed (Obs) values for 24 combinations tested experimentally. Red line represents the identity line y = x; blue line is the linear regression line. The linear regression slope was 0.92, with R2 = 0.93