Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: USP6NL mediated by LINC00689/miR-142-3p promotes the development of triple-negative breast cancer

Fig. 1

USP6NL exerts pro-oncogenic functions in TNBC by affecting cell proliferation, apoptosis, migration, invasion and EMT. a. The expression of USP6NL in TNBC cell lines and human normal breast cell line MCF-10A were respectively assessed with RT-qPCR. b. The RT-qPCR analyzed the interference efficiency of USP6NL in both cells transfected with sh-USP6NL#1/2. c. Colony formation assay was conducted to measure the change of cell proliferation in cells with or without USP6NL inhibition. d. Flow cytometry analysis determined the apoptosis rate of USP6NL-silenced cells. The X axis of the FACS dot plots meant cells labelled with FITC and the Y axis indicated cells dyed by PI. e-f. JC-1 assay and TUNEL assay were adopted to examine cell apoptosis under USP6NL interference or not. g-h. Transwell assay was used to assess the migration and invasion of TNBC cells with silenced USP6NL or not. i. EMT process in TNBC cells was also examined by IF analysis of E-cadherin and N-cadherin staining. *P < 0.05, **P < 0.01

Back to article page