Fig. 6

Nootkatone controls cyclin D1 at the protein level. a HCT-116 and SW480 cells were pre-treated with 100 μM of nootkatone for 1 h and exposed to 20 μg/ml of puromycin (Puro) at different time points. The cell lysate was harvested at each time point, wherein cyclin D1 and β actin protein levels were detected. Quantitative analysis was performed by Image J. The bottom graph represents degradation of the cyclin D1 protein over time. β-actin was used as loading control. b HCT-116 and SW480 cells were pre-treated with DMSO, 10 μM of MG132, or 0.1 μM of epoxomicin, followed by treatment with 50 μM (HCT-116) or 100 μM (SW480) nootkatone for 24 h. β-actin was used as loading control. Quantitative analysis was performed by Image J