Fig. 3

ERCC6L silencing induces cell cycle arrest and apoptosis in HCC cells. a. Cells were transfected with shERCC6L and NC for 24 h. Cell cycle progression was analyzed by PI staining with flow cytometry. The percentage of cells in each stage was quantified. Figures are representative of at least 3 independent experiments. Statistical differences were compared with NC, *p < 0.05. b. After transfected with shERCC6L and NC for 24 h, apoptosis cells were analyzed by Annexin V/FITC and PI staining with flow cytometry and apoptotic rates were quantified. Figures are representative of at least 3 independent experiments. c. Caspase 3/7 activation in SMMC7721 and HuH7 cells 24 h after transfection with shERCC6L or NC. Data are represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.001, indicates significant differences compared to the NC group