Fig. 2

Effect of knockdown ERCC6L expressin on HCC cells proliferation and migration in vitro. a. ERCC6L protein expression in SMMC7721 and HuH-7 cells transfected with shERCC6L or NC, as determined by Western blotting, *p < 0.05. b. ERCC6L mRNA expression was determined by RT-PCR. *p < 0.05. c. Proliferation of SMMC7721 and HuH-7 cells transfected with shERCC6L or NC, as determined by MTT assays, *p < 0.05 compared with NC at the same time point. d The clone formation assay detected cell proliferation after transfecting with ERCC6L shRNA. e. Migration of SMMC7721 and HuH-7 cells transfected with shERCC6L or NC, as determined by Transwell assays, *p < 0.05 compared with the respective NC group. Dot plots represent one of three independent experiments. In the histogram, data are mean ± SD of three experiments