Fig. 5From: Molecular evidence of IGFBP-3 dependent and independent VD3 action and its nonlinear response on IGFBP-3 induction in prostate cancer cellsIGFBP-3–independent reduction of Bcl-2 protein expression induced by VD3. a Western blotting image of Bcl-2 reduction by VD3 treatment. The ratio indicates the density of Bcl-2 band normalized by corresponding βActin band. b Effect of IGFBP-3 suppression on Bcl-2 reduction by VD3 treatment. The cells were infected with lentivirus encoding of shRNA for Igfbp-3, and then treated with VD3 and DHT: (top) quantification graph of Bcl-2 mRNA expression and (bottom) western blotting image of Bcl-2 and IGFBP-3 induced by VD3 and DHT treatment. The ratio indicates the density of Bcl-2 band normalized by corresponding βActin band. c Effect of IGFBP-3 overexpression on Bcl-2 reduction by VD3 treatment. The cells were infected with lentivirus encoding of the Cyp24a1 gene; then, the cells were treated with VD3 and DHT: (top) quantification graph of Bcl-2 mRNA expression and (bottom) western blotting image of Bcl-2 and IGFBP-3 induced by VD3 and DHT treatment. The ratio indicates the density of Bcl-2 band normalized by corresponding βActin band. The all experiments were performed in serum-containing medium condition. The uncropped full-length blot images are presented in Supplementary Fig. 5D, EBack to article page