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Fig. 3 | BMC Cancer

Fig. 3

From: Molecular evidence of IGFBP-3 dependent and independent VD3 action and its nonlinear response on IGFBP-3 induction in prostate cancer cells

Fig. 3

VD3 enhanced cytotoxicity of docetaxel. a Dose-dependent cytotoxicity of docetaxel. LNCaP cells were treated with docetaxel for 3 days, and then the living cell number was measured. IC50 was 0.82 nM. b Simultaneous treatment of VD3 with dose-dependent docetaxel. Simultaneous treatment of VD3 with docetaxel reduced the living cell number at 0.1–0.5 nM range. c IGFBP-3 overexpression was conducive to DHT/VD3 treatment on docetaxel treatment. IGFBP-3–overexpressed or control cells were treated with DHT/VD3 and 0.1 nM of docetaxel and cultured for 3 days, and then, the cell number was measured. d IGFBP-3 knockdown canceled the effect of VD3 on docetaxel treatment. In IGFBP-3 knockdown cells, DHT/VD3 treatment increased the cell number compared to that of the no-treatment control, and the cell number was almost equal to that of the DHT treated sample. The concentration of docetaxel used was 0.1 nM. The all experiments were performed in serum-containing medium condition

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