Table 1 Primer sequences used for qRT-PCR in this study
From: MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma
Gene | Forward primer | Reverse primer | Amplicon size |
|---|---|---|---|
MEIS1 | ATGACACGGCATCTACTCGTTC | TGTCCAAGCCATCACCTTGCT | 105 |
BMI1 | CGTGTATTGTTCGTTACCTGGAGAC | CATTGGCAGCATCAGCAGAAGG | 204 |
SALL4 | CCAGGGAATGACGAGGTGG | GAACTCCGCACAGCATTTCTC | 96 |
KLF4 | TCTTCTCTTCGTTGACTTTG | GCCAGCGGTTATTCGG | 210 |
OCT4 | GAACATGTGTAAGCTGCGGCC | CCCTTCTGGCGCCGGTTAC | 148 |
HIWI | ATGATTGAAGTGGATGACAGAACTG | TACTTGACAACAGACAGACAACTAT | 97 |
GAPDH | GGAAGGTGAAGGTCGGAGTCA | GTCATTGATGGCAACAATATCCACT | 101 |
NANOG | GGCAATGGTGTGACGCAGAAGGC | GCTCCAGGTTGAATTGTTCCAGGTC | 137 |
PLK1 | ATAGAGCGTGACGGCACTGAGT | TGCTCGCTCATGTAATTGCG | 107 |