Fig. 4

WDHD1 activates p-Akt expression to modulate cell cycle progression by up-regulating GCN5 in HPV E7 expressing cells. a The steady state levels of p-Akt by Western blot after GCN5 interference in RPE1 cells. b The steady state levels of p-Akt and GCN5 by Western blot after WDHD1 interference in RPE1 cells. c RPE1-E7 expressing cells were transfected with Flag-GCN5 or flag after WDHD1 siRNA transfection. The steady-state levels of p-Akt and GCN5 were measured by Western blotting. Tubulin was used as a loading control. Right panel, quantification of relative p-Akt levels from 3 independent experiments. Error bars reflect the standard deviations of the mean. NC, negative control. *P < 0.05; **P < 0.01. The WB samples for quantitative comparisons on different gels/blots derive from the same experiment and that gels/blots were processed in parallel. Cropping is used for the gels and blots in the main paper and the ‘full-length blots/gels are presented in Supplementary Figure 4