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Fig. 6 | BMC Cancer

Fig. 6

From: OTX1 exerts an oncogenic role and is negatively regulated by miR129-5p in laryngeal squamous cell carcinoma

Fig. 6

OTX1 acted as a direct target of the miR-129-5p in LSCC cells. a miR-129-5p could target OTX1 by using the human miRNA targets prediction tool miRanda and TargetSan. b Sequences of miR-129-5p and the potential binding sites in the 3’UTR of OTX1 is shown. c The expression of OTX1 was detected in Hep2 and TU212 cells using qRT-PCR after the transduction of miR-129-5p mimics. d The expression of OTX1 was detected in Hep2 and TU212 cells using Western blot after the transduction of miR-129-5p mimics. e, f Luciferase activity was determined in Hep2 and TU212 cells cotransfected with miRNAs (control mimics or miR-129-5p mimics) and a reporter vector containing miR-129-5p segments (WT or MUT) that bind to OTX1. **P < 0 .01

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