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Fig. 3 | BMC Cancer

Fig. 3

From: OTX1 exerts an oncogenic role and is negatively regulated by miR129-5p in laryngeal squamous cell carcinoma

Fig. 3

Knockdown OTX1 expression suppresses LSCC proliferation and colony formation in vitro. a OTX1 shRNAs inhibited the mRNA expression of OTX1 in Hep2 and TU212 cells using qRT-PCR. b OTX1 shRNAs inhibited the protein expression of OTX1 in Hep2 and TU212 cells using Western blot. c MTT assays showed that OTX1 downregulation inhibited the proliferation in Hep 2 cells. d MTT assays showed that OTX1 downregulation inhibited the proliferation in TU212 cells. e A colony formation assay demonstrated that OTX1 downregulation decreased the colony formation capacity of Hep2 and TU212 cells. f The quantitative graph of colony formation assay was performed to determine the colony formation capacity of Hep2 and TU212. SC: scrambled shRNA, #1: shOTX1–1, #2: shOTX1–2. *P < 0.05; **P < 0.01

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