Table 2 Antibodies used in immunofluorescence study
Antibody | Host species | Catalogue number and company | Dilution used |
|---|---|---|---|
ERCC1 | Mouse | Ab2356, Abcam | 1:98 |
Anti-betaIII | Rabbit | Ab18207, Abcam | 1:180 |
Anti-TIMP2 antibody [3A4] | Mouse | Ab1828, Abcam | 1:20 |
MT1-MMP | Rabbit | 13130S, Cell signaling | 1:200 |
OCT4 | Rabbit | Ab19857, Abcam | 1:180 |
EpCAM (VU109) | Mouse | 2929, Cell signaling | 1:800 |
STAT3 (124H6) (total STAT3) | Mouse | 9139S, Abcam | 1:1600 |
Phospho-Stat3 (Tyr705) | Mouse | 9131S, Cell signaling | 1:200 |
MMP2 | Goat | AF902, R&D systems | 1:40 |
CD44 (T2-F4) | Rat | Ab40983, Abcam | 1:100 |
CD133/Prom1 | Rabbit | Ab19898, Abcam | 1:200 |
Alexa Fluor™ 488 Goat Anti-Rabbit IgG (H+L) | Goat | A32731, Life Technology | 1:200 |
Alexa Fluor™ 568 Donkey Anti-Mouse IgG (H+L) | Donkey | A10037, Life Technology | 1:200 |
Alexa Fluor™ 594 Donkey Anti-Goat IgG (H+L) | Donkey | A32758, Life Technology | 1:200 |
Alexa Fluor™ 488 Goat Anti-Rat IgG (H+L) | Goat | A-11006, Life Technology | 1:200 |