Fig. 7
Effect of paclitaxel (pac) and cisplatin (cis) on the IC50 values and the expression of TIMP-2, MT1-MMP, MMP-2 in Cont and T2-KD cells. a Cell lines (FT282, JHOS2, and OVCAR4) were treated for 48 h with varying concentrations of either cisplatin or paclitaxel and their IC50 values (the concentration that kills 50% of the cells) was determined by MTT assay. T2-KD are TIMP-2 siRNA transfected cells (red line), P are parental cells without siRNA treatment (green line) and Cont are parental cells transfected with scrambled siRNA (blue line). Graphs are representative of three experiments done in triplicate. b-c The protein (by immunofluorescence) and mRNA (by qRT-PCR) expression of TIMP-2 (red), MTI-MMP (green) and MMP-2 (red) and co-stained DAPI (blue) in OVCAR4 Cont and T2-KD cells treated with their respective IC50 concentrations of cisplatin (red bars) and paclitaxel (blue bars). Images represent three independent experiments done in triplicate. For immunofluorescence images, magnification (20X); scale bar (in yellow) 20 μM. Significance was determined by one-way ANOVA *p > 0.05; **p > 0.01; ***p > 0.001; ****p < 0.0001 compared to Cont cells. *, **, **** in red significance obtained using an unpaired T-test (assuming Gaussian distribution) between Cont (untreated) and T2-KD (untreated) only