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Fig. 4 | BMC Cancer

Fig. 4

From: TIMP-2 regulates proliferation, invasion and STAT3-mediated cancer stem cell-dependent chemoresistance in ovarian cancer cells

Fig. 4

Expression of MT1-MMP, MMP-2 following reduction of TIMP-2 expression in cell lines. The protein expression of a MT1-MMP and b MMP-2 was evaluated by immunofluorescence in FT282, JHOS2 and OVCAR4 cell lines 48 h after siRNA knock down of TIMP-2 as described in Methods. T2-KD is representative of a pool of all three TIMP-2 siRNAs at a 3 nM final concentration. P are the parental cells treated with transfection reagent without siRNA treatment. Cont are parental cells transfected with scrambled siRNA. Images are representative of merged DAPI (blue) and MT1-MMP (green) or DAPI (blue) and MMP-2 (red) staining on individual cells performed on three passages in triplicates. The fluorescent intensity was obtained using FIJI software. Images represent three independent experiments, 20X magnification; scale bar (in yellow) 20 μM. Values are mean + SEM with significance deduced by using One-way ANOVA and indicated by **p < 0.01, ***p < 0.001; ****p < 0.0001). c MMP-2 activity in conditioned medium of OVCAR4 and JHOS2 cell lines was analysed by zymography as described in Methods. The treatment groups are T2-KD, Cont, P, Unt (untreated cells), +ve Control (serum conditioned media from HEY ovarian cancer cell line), −ve Control (serum reduced media, OPTIMEM). The image is representative of two experiments

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