Fig. 2

Knockdown and overexpression of USP18. a & b The relative mRNA and protein levels of USP18 were upregulated in C-33A, Caski, and SiHa cervical cancer cells, Three replications were analysed for each analysis. *** p < 0.001 vs HcerEpic. The full-length gels are presented in Supplementary Figure 2B. c & d USP18 siRNAs (siUSP18–1, siUSP18–2, and siUSP18–3) significantly suppressed the endogenous mRNA and protein levels of USP18 in SiHa and Caski cells. *** p < 0.001 vs siNC. Three replications were analysed for each analysis. The full-length gels are presented in Supplementary Figure 2D-up and Figure 2D-down. e & f oeUSP18 significantly improved the ectopic mRNA and protein levels of USP18 in HcerEpic cells. *** p < 0.001 vs oeNC. Three replications were analysed for each analysis. The full-length gels are presented in Supplementary Figure 2F