The presence of tumour-infiltrating lymphocytes (TILs) and the ratios between different subsets serve as prognostic factors in advanced hypopharyngeal squamous cell carcinoma

Table 2 Associations between the clinicopathological factors of HPSCC with the status of CD8, CD4, and Foxp3 infiltration and the CD8/Foxp3 and CD8/CD4 ratios (N = 132)

Characteristic	CD8		P	CD4		P	Foxp3		P	CD8/Foxp3		P	CD8/CD4		P
Characteristic	low	high	P	low	high	P	low	high	P	low	high	P	low	high	P
Age at diagnosis			0.033*			0.295			0.727			0.166			0.861
< 60y	31	40		31	40		37	34		33	38		34	37
≥ 60y	38	23		33	28		29	32		36	25		31	30
Sex			1.000			1.000			1.000			1.000			0.492
Male	1	0		0	1		0	1		1	0		1	0
Female	68	63		64	67		66	65		68	63		64	67
Smoke history			0.124			0.120			0.604			0.124			0.072
No	12	5		5	12		7	10		12	5		12	5
Yes	57	58		59	56		59	56		57	58		53	62
Drink history			0.671			0.834			0.832			1.000			0.832
No	16	12		13	15		13	15		15	13		13	15
Yes	53	51		51	53		53	51		54	50		52	52
Site			0.064			0.290			0.059			0.433			0.948
Pyriform sinus	57	59		54	62		54	62		59	57		57	59
Not pyriform sinus	12	4		10	6		12	4		10	6		8	8
Grade			0.003*			0.037*			0.003*			0.834			0.835
G1 + G2	61	42		55	48		59	44		53	50		50	53
G3	8	21		9	20		7	22		16	13		15	14
Stage			0.238			0.554			0.048*			0.609			1.000
III	15	20		15	20		12	23		17	18		17	18
IVA/IVB	54	43		49	48		54	43		52	45		48	49
T category			0.205			0.589			0.046*			0.449			0.718
T1–3	40	44		39	45		36	48		46	38		40	44
T4a	29	19		25	23		30	18		23	25		25	23
N category			1.000			0.599			0.289			0.536			0.860
N0–1	29	26		25	30		24	31		27	28		28	27
N2–3	40	37		39	38		42	35		42	35		37	40
Laryngectomy			0.379			0.599			0.480			0.725			0.295
Total	43	34		39	38		41	36		39	38		41	36
Partial	26	29		25	30		25	30		30	25		24	31
CD8 (cut off: 80)						0.001*			< 0.001*			0.001*			0.039*
Low				43	26		50	19		46	23		40	29
High				21	42		16	47		23	40		25	38
CD4 (cut off: 30)			0.001*						< 0.001*			0.163			< 0.001*
Low	43	21					49	15		29	35		15	49
High	26	42					17	51		40	28		50	18
Foxp3 (cut off: 30)			< 0.001*			< 0.001*						0.014*
Low	50	16		49	17					27	39		29	37	0.296
High	19	47		15	51					42	24		36	30

Abbreviations: HPSCC hypopharyngeal squamous cell carcinoma, G1 Well differentiated, G2 Moderately differentiated, G3 Poorly differentiated
*The P value is significant

ISSN: 1471-2407