Fig. 1

Assessing the anti-cancer effects of Etanercept as a TNF-α blocker. a MTT viability test in MDA-MB-231 cells following treatment of cells with 1 or 10 μg/ml Etanercept. Data represent mean ± sem for six replicates. ME1 and ME10 = MDA-MB-231 + 1 or 10 μg/ml Etanercept. b Analysis of cell cycle in MDA-MB-231 cells treated with 1 or 10 μg/ml Etanercept. Data represent mean ± sem of number of cells in each phase for 3 replicates. c Measurement of apoptosis using annexin-PI. Data represent mean ± sem of number of cells in each phase for 3 replicates. d Measurement of NFKB activity following treatment with Etanercept. Data are normalized to the activity of NFKB in MDA-MB-231 cells as control. ^*P < 0.05 ^**P < 0.01, ^***P < 0.0001 . ME1 and ME10 = MDA-MB-231 + 1 or 10 μg/ml Etanercept