Fig. 2

Increased transmembrane migration and invasion of cryopreserved breast cancer cells. a ZR-75-1 cells of the non-intervened group/before cryopreservation and the investigated group/after cryopreservation were seeded in transwell and cultured for 72 h. The number of stained cells on the bottom of the membrane after cryopreservation was significantly higher than before cryopreservation. b MDA-MB-231 cells of the group before and after cryopreservation were seeded in transwell and cultured for 8 h. The number of stained cells on the bottom of the membrane after cryopreservation was significantly higher than before cryopreservation. c and d Triplicate samples of each group from three independent experiments were included (n = 3). Data are analyzed using Student’s t-test, and expressed as mean ± SD. Magnification × 13.5. Significantly different at ^***p < 0.001