Fig. 4

Representative examples of immunoexpression patterns of MIB-1, TEX19 and Beta-catenin. a – Two distinct pure seminoma cases, one with diffuse nuclear staining for MIB-1 (on the left) and another with < 50% positive tumor cells (on the right); b – A case of pure embryonal carcinoma with 100% positivity for MIB-1; c – Faint cytoplasmic positivity for TEX19 in a pure yolk sac tumor with hyaline globules; d – Complete absence of staining for TEX19 in a pure seminoma; e – Two distinct pure seminoma cases, one with diffuse, strong, membrane staining for Beta-catenin (on the left) and another with very faint and discontinuous membrane staining in 20% of tumor cells, rendering a combined score ≤ 100 (on the right). Notice the internal positive control (staining in capillary vessels within the tumor); f – Strong and diffuse membrane staining for Beta-catenin in a mixed tumor composed of yolk sac tumor, embryonal carcinoma and teratoma, rendering a combined score > 100