Fig. 3

Immunohistochemistry analysis of AR and comparison with qRT-PCR positivity. IHC was carried out in the tumor specimens (n = 80) using anti human mouse monoclonal AR antibody and the percentage of nuclear stained cells were graded for AR positivity. Representative images were shown for strong AR expression (a), weak expression (b) and negative staining (c). Bar graph showing the number of cases assessed for AR expression by two methods (IHC and qRT-PCR) of detection (d). The whisker plot showing a comparison of the delta Cq values of AR IHC (+) tumors and AR IHC (−) tumors. AR IHC (+) cases had low delta Cq values indicating high AR mRNA expression and AR IHC (−) cases had high delta Cq values indicating a low expression of AR (e). All the images were captured with a magnification of 100x