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Fig. 3 | BMC Cancer

Fig. 3

From: Breast cancer diagnosis is associated with relative left ventricular hypertrophy and elevated endothelin-1 signaling

Fig. 3

Breast cancer conditioned medium induces hypertrophy in human cardiomyocytes through endothlin-1 signaling pathway. a Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 μm, b Quantification of cell surface area in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM with or without the antibody against ET-1 (n = 70 per group). c β-MHC mRNA levels that were normalized to β-ACTIN in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM with or without antibodies against ET-1 (n = 6 per group), d Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 μm, e Quantification of cell surface area levels in cardiomyocytes treated with either regular serum free medium and breast cancer MCF7 CM with or without BQ-1 (n = 70 per group), f Cardiomyocytes were stained with WGA (green) and DAPI (blue), scale bar = 50 μm, g Quantification of cell surface area in cardiomyocytes treated with regular serum free medium, synthetic ET-1, breast cancer ZR75–1 CM or breast cancer T47D CM. h β-MHC mRNA levels that were normalized to β-ACTIN in cardiomyocytes treated with regular serum free medium, synthetic ET-1, breast cancer ZR75–1 CM or breast cancer T47D CM (n = 6 per group). i Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 μm, j Quantification of cell surface area in cardiomyocytes treated with regular serum free medium or triple negative breast cancer MDA-MB-231 CM. Results are shown as means ± SEM. Comparisons between two groups were made by unpaired t-test, whereas comparisons between three groups were made by one-way ANOVA with a Tukey Kramer’s post hoc multiple comparison test. + p < 0.05 vs its own control group. * p < 0.05 vs its CM

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