Fig. 2

Human breast cancer conditioned medium induces hypertrophy in human left ventricular cardiomyocytes. a Schematic of conditioned medium (CM) treatment in human left ventricular cardiomyocytes. b Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 μm, c Quantification of cell surface area in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM (n = 70 per group). d β-myosin heavy chain (β-MHC) mRNA levels that were normalized to β-ACTIN in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM (n = 6). e Lysates from cardiomyocytes were immunoblotted with antibodies against phosphorylated eukaryotic elongation factor 2 (p-eEF2), total-eEF2 and β-ACTIN, full blot is provided in supplementary file, f Quantification of protein expression levels in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM (n = 4 per group). g Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 μm, h Quantification of cell surface area in cardiomyocytes treated with either regular serum free medium or low molecular weight fractionated medium (LMWF, < 30 kDa) or high molecular weight fractionated medium (HMWF, > 30 kDa) (n = 70 per group). i Quantification of endothelin-1 (ET-1) levels in cardiomyocytes treated with fractionated breast cancer MCF7 CM (n = 6 per group). Results are shown as means ± SEM. Comparisons between two groups were made by unpaired t-test, whereas comparisons between three groups were made by one-way ANOVA with a Tukey Kramer’s post hoc multiple comparison test. + p < 0.05 vs its own control group. Figure 2A was modified from Servier Medical Art, licensed under a Creative Common Attribution 3.0 Generic License. http://smart.servier.com/