Fig. 1

Knockdown of mDia1, mDia2, FHOD1, and INF2 expression in U87, U138, and primary glioblastoma cell lines UTGB7 and T86. a The knockdown efficacy was examined 48 h after transfection by immunoblotting. GAPDH or tubulin was used as a control for protein loading. Representative immunoblots have been cropped from repeated experiments to save space. The full-length blots are presented in Supplement 2. b) Representative images from spheroid migration experiment using the T86 primary cell line. Timepoints 0 and 48 h are shown. The edge of migrating cells is drawn. c) Plots showing fold increase of areas (FI) as a function of time (images were taken every 2 h). Bar graphs highlight the FI in individual treatment groups at 48 h. Asterisks indicate significant reduction of migration: * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001